Table 5 Oligonucleotides used in this study
Assay | Name | Oligonucleotide | Position (nt) | Sequence (5′-3′) | Location |
|---|---|---|---|---|---|
Pan-Simbu PCR | panOBV-L-2959 F | Primer | 2888-3167 | TTG GAG ART ATG ARG CTA ARA TGT G | L-Segment |
panOBV-L-3274R | Primer | TGA GCA CTC CAT TTN GAC ATR TC | |||
SBV-M1 | SBV-M1-213 F | Primer | 1690-1827 | TCA ATT CAG CAA GTA ACA TAC AAT GG | M-Segment |
SBV-M1-350R | Primer | CGT GGT CTG TCT TGG TTG ATG | |||
SBV-M1-240FAM | Probe | FAM-AAG CAC TGG CCC GAA GTT TCA CCT-BHQ1 | |||
SBV-L1 | SBV-L1-11 F | Primer | 367-511 | TTG CCG TTT GAT TTT GAA GTT GTG | L-Segment |
SBV-L1-155R | Primer | TCA GGG ATC GCA AAT TAA AGA ACC | |||
SBV-L1-36FAM | Probe | FAM-TCA TCC GTG CTG ACC CTC TGC GAG-BHQ1 | |||
SBV-L1.4 | SBV-L1.2 F | Primer | 361-468 | TCA GAA TTG CCG TTT GAT TTT GAA G | L-Segment |
SBV-L1.4R | Primer | GTT GAG CGG CCC AAA TAT TTC C | |||
SBV-L1-36FAM | Probe | FAM-TCA TCC GTG CTG ACC CTC TGC GAG-BHQ1 | |||
SBV-S3* | SBV-S-382 F | Primer | 382-469 | TCA GAT TGT CAT GCC CCT TGC | S-Segment |
SBV-S-469R | Primer | TTC GGC CCC AGG TGC AAA TC | |||
SBV-S-408FAM | Probe | FAM-TTA AGG GAT GCA CCT GGG CCG ATG GT-BHQ1 |