Table 3 Comparison of pan-Simbu real-time RT-PCR and SBV diagnostic PCRs using field samples
Pan Simbu | SBV assays | |||||||
|---|---|---|---|---|---|---|---|---|
Sample | Material | Species | Origin | M1 | L1 | L1.4 | S3 | |
1 | Brain | Sheep (lamb) | NRW | 34.1 | 27.2 | 27.2 | 26.7 | 23.7 |
2 | Cerebellum | Goat (goatling) | NRW | 32.3 | 26.3 | 25.5 | 26.3 | 22.5 |
3 | Med. obl. | Goat (goatling) | NRW | 36.0 | 31.0 | 28.3 | 29.1 | 25.5 |
4 | Brain | Goat (goatling) | NRW | 34.4 | 28.3 | 24.5 | 24.9 | 21.5 |
5 | Inter. Candolis | Goat (goatling) | NRW | 33.2 | 27.3 | 25.5 | 26.2 | 22.6 |
6 | Brain | Sheep (fetus) | NRW | 39.3 | 38.2 | 37.8 | 39.4 | 33.9 |
7 | Blood | Sheep (lamb) | NRW | 38.1 | 36.6 | 35.4 | 36.0 | 32.2 |
8 | Med. obl. | Sheep (lamb) | NRW | 35.4 | 28.0 | 29.9 | 30.8 | 27.1 |
9 | Cerebellum | Sheep (lamb) | NRW | 38.3 | 34.8 | 34.3 | 34.7 | 31.7 |
10 | Cerebrum | Sheep (lamb) | NRW | 37.0 | 32.6 | 32.2 | 33.0 | 31.4 |
11 | Spinal cord | Sheep (lamb) | NRW | 36.7 | 30.5 | 30.6 | 31.7 | 28.1 |
12 | Blood | Sheep (lamb) | NRW | neg. | 32.0 | neg. | 36.1 | 31.9 |
13 | Med. obl. | Sheep (lamb) | NRW | 37.2 | 32.6 | 32.1 | 33.0 | 28.3 |
14 | Cerebellum | Sheep (lamb) | NRW | 38.7 | 31.4 | 33.8 | 35.5 | 30.4 |
15 | Cerebrum | Sheep (lamb) | NRW | 36.6 | 30.7 | 29.9 | 30.5 | 26.3 |
16 | Cerebrum | Sheep (lamb) | NRW | 32.6 | 27.0 | 25.4 | 26.3 | 23.1 |
17 | Med. obl. | Sheep (lamb) | NRW | 39.0 | 34.2 | 37.2 | 38.6 | 32.3 |
18 | Cerebrum | Sheep (lamb) | NRW | 29.7 | 22.6 | 22.5 | 21.3 | 16.8 |
19 | Cerebrum | Sheep (lamb) | NRW | 39.9* | neg. | neg. | 39.2* | 37.0 |
20 | EDTA blood | Sheep | Hesse | 38.8* | 34.8 | 34.5 | 34.7 | 30.3 |
21 | EDTA blood | Sheep | Hesse | neg. | 38.6 | 37.4 | 37.3 | 34.7 |
22 | Organ material | Sheep | Hesse | 28.9 | 25.5 | 20.3 | 20.1 | 15.2 |
23 | Cerebrum | Sheep (lamb) | Hesse | 38.1 | 29.8 | 31.0 | 31.7 | 28.1 |
24 | Brain stem | Sheep | LS | 36.4 | 32.4 | 28.7 | 29.3 | 26.4 |
25 | Cortex | Sheep | LS | 36.5 | 33.2 | 30.1 | 30.7 | 27.5 |
26 | Brain | Sheep (lamb) | LS | 38.0 | 32.9 | 31.3 | 31.1 | 28.6 |
27 | Brain | Goat (goatling) | BW | 31.9 | 25.8 | 21.7 | 22.1 | 18.1 |
28 | Serum | Goat (goatling) | BW | 39.6* | 35.5 | 32.0 | 32.4 | 28.8 |
29 | Cruor | Goat (goatling) | BW | 34.1 | 30.2 | 28.5 | 29.0 | 25.5 |
30 | Cerebellum | Sheep | SA | 34.1 | 31.1 | 30.6 | 30.4 | 28.0 |
31 | Cerebrum | Sheep | SA | 36.7 | 32.7 | 31.7 | 31.8 | 29.2 |
32 | Cerebrum | Sheep | SA | 29.8 | 25.3 | 22.2 | 22.4 | 21.8 |
33 | Brain | Sheep (fetus) | Bavaria | 33.7 | 30.2 | 25.0 | 24.6 | 22.0 |
34 | Brain | Sheep (fetus) | Bavaria | neg. | 33.8 | 36.0 | 36.7 | 32.9 |
35 | EDTA blood | Sheep (fetus) | Bavaria | neg. | 36.2 | neg. | 38.7* | 34.9 |
36 | Organ material | Sheep (lamb) | HH | neg. | neg. | neg. | neg. | neg. |
37 | Organ material | Sheep | HH | 36.5 | 26.4 | 27.5 | 27.3 | 25.4 |
38 | Organ material | Sheep (lamb) | SH | 38.9 | 32.9 | 31.2 | 30.7 | 27.7 |
39 | Serum | Cattle | TH | neg. | neg. | neg. | neg. | neg. |
40 | Cerebrum | Bison (fetus) | RP | 29.4 | 22.2 | 21.1 | 21.6 | 15.8 |
41 | Cerebrum | Sheep | BB | neg. | 33.6 | 32.3 | 32.7 | 28.2 |
42 | Blood | Cattle | animal trial | 29.7 | 20.4 | 21.9 | 22.9 | 21.1 |
43 | Blood | Sheep (lamb) | NRW | neg. | neg. | neg. | neg. | neg. |
44 | Med. obl. | Sheep (lamb) | NRW | neg. | neg. | neg. | neg. | neg. |
45 | Cerebellum | Sheep (lamb) | NRW | neg. | neg. | neg. | neg. | neg. |
46 | Cerebrum | Sheep (lamb) | NRW | neg. | neg. | neg. | neg. | neg. |
47 | Spleen | Sheep (lamb) | NRW | neg. | neg. | neg. | neg. | neg. |
48 | Muscle | Sheep (lamb) | NRW | neg. | neg. | neg. | neg. | neg. |