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Table 2 Evaluation of the sensitivity of the individual assays using an SBV-RNA dilution series

From: Development of a pan-Simbu real-time reverse transcriptase PCR for the detection of Simbu serogroup viruses and comparison with SBV diagnostic PCR systems

 

Pan Simbu

SBV assays

Sample*

 

M1

L1

L1.4

S3

SBV 10-2

30.6

22.5

23.3

23.6

21.9

SBV 10-3

34.5

26.1

26.8

26.6

24.5

SBV 10-4

38.2

29.2

30.1

30.1

27.8

SBV 10-5

neg.

32.6

33.3

33.3

31.1

SBV 10-6

neg.

41.1#

36.4

36.8

34.6

SBV 10-7

neg.

neg.

neg.

39.6#

37.8

SBV 10-8

neg.

neg.

neg.

neg.

neg.

  1. *log10 dilution series of SBV RNA extracted from cell culture sample BH80/11-4 (KC/BHK), initial titer 1× 106.3 TCID50/ml; #only one of the duplicates positive; neg.: negative result; pan Simbu: pan-Simbu real-time RT-PCR system; M1: SBV-M1 assay; L1: SBV-L1 assay, L1.4: SBV-L1.4 assay; S3: SBV-S3 assay; columns 2–6 represent mean Cq-values from duplicates.
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Virology Journal

ISSN: 1743-422X