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Table 1 PCR analysis of Simbu serogroup viruses and Bunyamwera serogroup viruses

From: Development of a pan-Simbu real-time reverse transcriptase PCR for the detection of Simbu serogroup viruses and comparison with SBV diagnostic PCR systems

    

Pan Simbu

SBV assays

Species*

Virus

Strain

TCID50/ml

 

M1

L1

L1.4

S3

Akabane

AKAV

OBE-1

1 × 105.4

33.7

neg.

neg.

neg.

neg.

Akabane

SABOV

AN 9398

1 × 102.5

29.3

neg.

neg.

neg.

neg.

Akabane

TINV

CSIRO 153

1 × 103.1

30.2

neg.

neg.

neg.

neg.

Bunyamwera

BATV

53.2

1 × 105

32.7

neg.

neg.

neg.

neg.

Bunyamwera

BUNV #

ATCC VR-87

1× 104.5

33.1

neg.

neg.

neg.

neg.

Bunyamwera

NRIV #

51

-

31.7

neg.

neg.

neg.

neg.

Oropouche

OROV

TRVL 9760

1 × 103.5

33.2

neg.

neg.

neg.

neg.

Sathuperi

SATV

l-11155

1 × 102

28.5

neg.

neg.

neg.

neg.

Sathuperi

DOUV

CSIRO 150

1 × 102.5

35.7

neg.

neg.

neg.

25.4

Sathuperi

SBV

BH80-11

1 × 104.3

30.6

22.5

23.3

23.6

21.9

Shamonda

SHAV

An 5550

1 × 102

30.6

neg.

30.0

23.9

22.2

Shamonda

PEAV

CSIRO 110

1 × 102

31.4

neg.

neg.

neg.

neg.

Shamonda

SANV

An 5077

1 × 103

30.1

neg.

neg.

neg.

neg.

Shuni

SHUV

An 10107

1 × 102.5

28.0

neg.

neg.

neg.

neg.

Shuni

AINOV

Ja N Ar 28

1 × 102

27.4

neg.

neg.

neg.

neg.

Simbu

SIMV

SA Ar 53

1 × 103

27.2

neg.

neg.

neg.

neg.

Thimiri

THIV

-

1 × 104.1

36.2

neg.

neg.

neg.

neg.

  1. neg.: negative result; -: not available; pan Simbu: pan-Simbu real-time RT-PCR system; TCID50: 50% tissue culture infectious doses; M1: highly specific SBV-M1 assay; L1: SBV-L1 assay, L1.4: SBV-L1.4 assay; S3: SBV-S3 assay; *species classification according to [10], SBV [7]; #1:100 dilution of original RNA; columns 5–9 represent mean Cq-values from duplicates.
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Virology Journal

ISSN: 1743-422X