Primer | Sequence (5′ → 3′)a | Locationsband modifications |
---|---|---|
PS7-6-F | CTAG ccatgg GA ATGAATTACACTTTAAGTG | 1aa; Nco I |
PS7-9-R | CG ggatcc tcacta TTAAGAATTCAGTATC | Full-length reverse primer with stop codon; Bam HI; |
PS7-14-F | GAACTC ggatcc ATGAATTACACTTTAAG | 1aa; Bam HI; |
PS7-15-R | CCG ctcgag TTAAGAATTC AGTATC | Full-length reverse primer with stop codon; Xho I; |
PS7-16-R | CCC aagctt tta GTGGTGGTGGTGGTGGTG AGAATTCAGTATCTTTTTG | Full-length reverse primer without stop codon; Hin dIII; six His were introduced; |
PS7-17-R | CCG ctcgag AGAATTCAGTATCTTTTTG | Full-length reverse primer without stop codon; Xho I; |
PS7-18-R | T GC AGGAACG TCGAATGAATC | 79aa; GC substitute for AA; |
PS7-19-F | G CTTTTGCTGAGTTGCTTGATC | 80aa; G substitute for C; |
TS7-23-F | ggatcc gtcgac ccatgg GA CCCGAAATTAATCTTGTC | 25aa; Bam HI, Nco I, Sal I; |
TS7-25-F | ggatcc gtcgac ccatgg GA GTGAATTTACTATCTGAT | 44aa; Bam HI, Nco I, Sal I; |
TS7-27-F | ggatcc gtcgac ccatgg GA TTACCTTTTGCTGAGTTG | 79aa; Bam HI, Nco I, Sal I; |
TS7-8-R | CG ggatcc ggtacc tcacta TTAAGAATTCAGTATCT | Full-length reverse primer with stop codon; Bam HI, Kpn I; |
TS7-21-F | ggatcc gtcgac ccatgg GA ATGAATTACACTTTAAGT | 1aa, Bam HI, Sal I, Nco I; |
TS7-12-R | CG ggatcc ggtacc tta ACTCTTAAATATCAAAG | 295aa; Bam HI, Kpn I; |
TS7-14-R | CG ggatcc ggtacc tta AAACAGAGAATACCAATAATC | 287aa; Bam HI, Kpn I; |
TS7-16-R | CG ggatcc ggtacc tta GGGTATCATACTTAATTTTCC | 214aa; Bam HI, Kpn I; |
MSKP1-1-F | CG ggatcc ATGGCCGCCGAGGGC | 1aa; Bam HI; |
MSKP1-2-R | CCG ctcgag CTCGAAGGCCCACTG | Full-length reverse primer without stop codon; Xho I; |
MSKP1-3-F | CG gaattc ATGGCCGCCGAGGGC | 1aa; Eco RI; |
MSKP1-4-R | CG ggatcc TACGTGCTTGTTGCAG | 67aa; Bam HI; |
MSKP1-5-F | CG gaatcc GAGGACCTCAAGAAC | 97aa; Eco RI; |
MSKP1-6-R | CG ggatcc ACCCTTGATGTTCAGATAG | 128aa; Bam HI; |
MSKP1-8-F | CCG ctcgag CT ATGGCCGCCGAGGG | 1aa; Xho I; |
MSKP1-9-R | CG ggatcc CTCGAAGGCCCACTG | Full-length reverse primer without stop codon; Bam HI; |
MSKP1-10-F | CCC aagctt CG ATGGCGGCCGAGGGCGAG | 1aa; Hin dIII; |