Skip to main content
Figure 1 | Virology Journal

Figure 1

From: Nonstructural protein P7-2 encoded by Rice black-streaked dwarf virus interacts with SKP1, a core subunit of SCF ubiquitin ligase

Figure 1

Strong interaction between P7- 2 and SKP1Maizein YTH system. Yeast colonies containing the combinations of pGBKT7-S7-2/pGADT7-SKP1Maize and pGBKT7-S7-2/pGADT7-SKP1rMaize grew well on the selective medium and turned blue, as did yeast colonies containing the positive control pGBKT7-p53/pGADT7-T. pGADT7-SKP1rMaize is the prey clone from the positive transformants obtained in the two-hybrid screens, harboring the mRNA sequence of SKP1, while pGADT7-SKP1Maize is the construct that expresses the exact SKP1Maize protein. Yeast transformed with pGBKT7-S7-2/pGADT7 or pGBKT7/pGADT7-SKP1Maize used as negative controls were unable to grow.

Back to article page

Virology Journal

ISSN: 1743-422X