5-Lipoxygenase expression by neutrophils incubated with or without dengue viruses. The proteins were isolated from neutrophils and were electrophoretically transferred from the gel onto Hybond-C Extranitrocellulose membrane. The membrane was then cut, based on the size of proteins of interest, to separate the 5-lipoxygenase (79 kDa) and the control protein GAPDH (39 kDa). (A) Representative films from 5-lipoxygenase and GAPDH Western blots performed with untreated neutrophils and neutrophils incubated with live (DENV-2) or heat-inactivated dengue viruses (hiDENV-2). (B) Expression of 5-lipoxygenase protein by untreated neutrophils (×) and neutrophils exposed to live (DENV-2, □) or heat-inactivated dengue viruses (hiDENV-2, ■) (N = 3). * p < 0.05 vs untreated cells using one-way ANOVA analysis of the area under the curves with Bonferroni adjustments.