Figure 3
From: Analysis of whole genome sequences of 16 strains of rubella virus from the United States, 1961–2009
Sequence variation among 30 rubella viruses. The percentage of nucleotide variability (A) and amino acid variability (B) in each domain, as denoted on the X-axis, of 30 rubella viruses relative to overall consensus sequence was calculated using Microsoft Office Excel. The Y-axis indicates the percentage of variability. The amino acid alignment was determined by ClustalW according to the Gonnet PAM 250 matrix. Panels (C and D) show the variation of nucleic acid and amino acid sequences for the 6 regions shown in Figure 2. The conserved, semi-conserved, or non-conserved designation of each aa position was enumerated based on the most variable aa that was observed at that position amongst the 30 viruses. The clade-specific variations in nucleic acid sequences are compared using clade-specific consensus sequences. The range of each domain is indicated at the bottom of the graphs.