CD4+ and CD8+ T-cell responses to HSV-1 vaccine antigens. Cells were stimulated with either HSV-1 gB or HSV-1 gD peptide ex vivo for 6 hrs and then stained for the detection of antigen-specific IFN-γ and TNF-α cytokine responses. The responses for each mouse for the specified antigen are shown in scattered plots. The cutoff value for IFN-γ and TNF-α cytokine responses were based on the data generated from the mock control mice. Percentages of responses greater than 0.03 are considered positive for the specified antigen and cytokine response. Media control values were subtracted from each value before the analysis.