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Figure 1 | Virology Journal

Figure 1

From: Induction of robust immunity response in mice by dual-expression-system-based recombinant baculovirus expressing the capsid protein of porcine circovirus type 2

Figure 1

Schematic representation of the structure ofBV-GD-ORF2. The ORF2 gene cassette consists of the gp64 signal sequence (SP), the ORF2 gene (ORF214–234) fused to the N-terminus of the AcMNPV major envelope protein gp64 gene (gp6425–517), and the poly(A) sequence, which was driven by a dual promoter containing the CMV immediate-early enhancer/promoter (pCMV-IE) and the polyhedrin promoter (PPH). The VSV-G expression cassette was driven by the p10 promoter (PP10). ORF214–234:ORF2 corresponding to amino acids 14–234, but lacking the N-terminal nuclear localization signal peptide of the capsid protein of PCV2. Gp6425–517: gp64 corresponding to amino acids 25–517.VSV/G: the glycoprotein of vesicular stomatitis virus.

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