Figure 7

Trans -activation of the HBoV1 promoter by the NS1 protein. (A) The luciferase activity of the construct pGL3-(1–252) in 293 T and HeLa cells in the presence of the NS1 expression. Cells co-transfected with pGL3-(1–252) and mNS1 constructs in the absence of the NS1 were used as controls. The relative luciferase activity was calculated as the ratio between the pGL3-(1–252) activity and the control plasmid pRL-TK activity. The error bars indicate standard deviations of three independent experiments and each was performed in triplicate. (B) Detection of the NS1 protein in cells co-transfected with pGL3-(1–252) and WT-NS1 constructs by Western blot assay. Actin was used as a loading control. WT-NS1, pGL3-pCMV-NS1; mNS1, pGL3-pCMV-NS1mut. The mean values and standard deviation (SD) of three independent experiments are shown (**: p < 0.01).