Figure 6From: Key elements of the human bocavirus type 1 (HBoV1) promoter and its trans-activation by NS1 proteinAnalysis of transcriptional activity of HBoV1 promoter in HeLa cells. Mutants for putative CREB binding site at nt 118 (pGL3-CREBmut), CdxA binding site at nt 146 (pGL3-CdxAmut), CAAT box at nt 131 (pGL3-CAATmut) and TATA box at nt 155 (pGL3-TATAmut) were obtained by PCR-based site-directed mutagenesis. The pGL3-Basic without any promoter was used as a negative control. The error bars indicate standard deviations of three independent experiments and each performed in triplicate. Black rhombic symbols, mutant sites; Ins, transcription initiator sequence.Back to article page