Comprehensive profiling of Epstein-Barr virus-encoded miRNA species associated with specific latency types in tumor cells

Table 1 Characterization of EBV coding and noncoding transcripts in cells and NPC tissues tested

Latency type	Cell line/tissue	Coding mRNA				Non-coding miRNAs
		LMP1	EBNA1	Promoter	Activity	BHRF1 family	BART family
			Cp	Wp	Qp
EBV-positive
I	Akata(+)(BL)	-	Low	Low	High	+	++
I/III	Daudi(BL)	+	Low	Medium	High	++	+
II	C666-1	+	None	Low	High	+	+++
	NPC tissues	+
III	Raji(BL)	+	None	Medium	Low	+	+/-
	Namalwa(BL)	+/-	None	Low	None	+/-	-
	B95-8(LCL)	++	None	High	Medium	++	+
EBV-negative
	CNE2(NPC)	-	-	-	-	-	-
	Ramos(BL)	-	-	-	-	-	-
	Akata(-)(BL)	-	-	-	-	-	-

Note: The abundance of all EBV latent coding and noncoding transcripts in the table above was determined through two different quantitative real time RT-PCR procedures respectively (see Materials and methods).
The abbreviation in the bracket indicates the tumor from which the cell lines were established with the exception to LCL. NPC: Nasopharyngeal carcinoma; BL: Burkitt’s lymphoma; LCL: Lymphoblastoid cell line.
-, negative; +/-,+,++ ,+++ indicate the level of EBV latent transcripts in an increasing order.

ISSN: 1743-422X