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Figure 4 | Virology Journal

Figure 4

From: Comprehensive profiling of Epstein-Barr virus-encoded miRNA species associated with specific latency types in tumor cells

Figure 4

The pattern of the EBV miRNA transcriptome in NPC cells and tissues in the latency II state. (A) RT-PCR analysis of the EBV microRNA transcriptome in the persistently EBV-positive cell line C666-1 and three NPC tissue samples. U6 was used as an internal control. The whole EBV miRNAome is grouped into four clusters based on genomic location in sequential order. (B) Spearman and Pearson correlations between the C666-1 cells and the three NPC tissue samples (left). The expression levels of the EBV miRNAs were quantified by real-time RT-PCR. The data shown in the lower left portion are Pearson’s correlation coefficients, and the values shown in the upper right portion are Spearman’s correlation coefficients. To calculate the correlations, the real-time PCR results for the NPC tissues and C666-1 cells were log2-transformed and analyzed using Person’s correlation analysis. Pearson’s correlation coefficients and p-values are shown. The average expression level of each EBV miRNA species in the three NPC tissues is plotted on the x-axis (right). (C) Proportions of the respective human and EBV miRNA species that were detectable in C666-1 cells (left) and two NPC samples (middle and right) based on the microarray analysis. The EBV-encoded miRNAs accounted for more than 10% of the entire human miRNA library in the NPC samples in vivo and in vitro.

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