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Figure 2 | Virology Journal

Figure 2

From: Comprehensive profiling of Epstein-Barr virus-encoded miRNA species associated with specific latency types in tumor cells

Figure 2

Characteristic expression patterns of EBV latency biomarkers for viral-coding genes. The levels of EBNA1 transcripts that were derived from promoters Q (Qp), C (Cp), and W (Wp) and the expression of LMP1 (latent membrane protein 1) were detected in cells having the three different EBV latency types by quantitative RT-PCR. Daudi and Akata(+) cells are EBV-positive BL (Burkitt’s lymphoma) cells with type I latency. T4, T5, T6 and T7 are pathologically confirmed, clinical NPC (nasopharyngeal carcinoma) specimens with EBV type II latency, and the C666-1 cell line is an epithelial NPC cell line that consistently harbors EBV. The Raji and Namalwa cell lines are EBV latency III BL cell lines, and the B95-8 cell line is a macaque lymphoblastoid cell line (LCL) harboring a prototype EBV strain. Akata(−), CNE2 and Ramos cells were used as EBV-negative controls, each qPCR reaction was run in triplicate, and the relative expression levels were calculated using the comparative method (2-ΔΔCt) with normalization to GAPDH.

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