Table 6 Primers and probes used in this study*
Primer Name | Sequence (5′ to 3′) | Target Gene | Reference |
|---|---|---|---|
Primers for real time RT-PCR | |||
Set 1 | |||
Inf A- F | GACCRATCCTGTCACCTCTGAC | M | [15] |
Inf A-R | AGGGCATTYTGGACAAAKCGTCTA | ||
Inf A- probe | TAMRA-TGCAGTCCTCGCTCACTGGGCACG-BHQ2 | ||
InfB-F | ATCGGATCCTCAACTCACTCTT | NS | [16] |
InfB-R | TGACCAAATTGGGATAAGACTC | ||
InfB-probe | FAM-CTCGAATTGGCTTTGRATGTCCTTCAT-BHQ1 | ||
FluC-F | TGCATTAAAAGCGGATTCGTT | NS | this study |
FluC-R | GCTGGGCTCCTGATTTGATACT | ||
FluC-probe | HEX-TGGCTACCGATGAAATCTCTCTCACTATACT-BHQ1 | ||
RNase P-F | AGATTTGGACCTGCGAGCG | RNase P | [15] |
RNaseP-R | GAGCGGCTGTCTCCACAAGT | ||
RNaseP-probe | CY5-TTCTGACCTGAAGGCTCTGCGCG-BHQ3 | ||
Set 2 | |||
RSV-F | GCCAAAAAATTGTTTCCACAATA | L | [16] |
RSV-R | TCTTCATCACCATACTTTTCTGTTA | ||
RSV- probe | FAM-TCAGTAGTAGACCATGTGAATTCCCTGCA-BHQ1 | ||
hMPV-F | AACCGTGTACTAAGTGATGCACTC | N | [17] |
hMPV-R | CATTGTTTGACCGGCCCCATAA | ||
hMPV-Probe | Cal Flour Orange560-CTTTGCCATACTCAATGAA CAAA CT-BHQ1 | ||
Rhino-F | TGGACAGGGTGTGAAGAGC | 5′- UTR | [18] |
Rhino-R | CAAAGTAGTCGGTCCCATCC | ||
Rhino-Probe | TAMRA-TCCTCCGGCCCCTGAATG-BHQ2 | ||
Adeno-F | GCCCCAGTGGTCTTACATGCACATC | H | [16] |
Adeno-R | GCCACGGTGGGGTTTCTAAACTT | ||
Adeno-Probe | Quasar670-TCGGAGTACCTGAGCCCGGGTCTGGTGCA-BHQ2 | ||
Set 3 | |||
SW InfA -F | GCACGGTCAGCACTTATYCTRAG | Np | [15] |
SW InfA-R | GTGRGCTGGGTTTTCATTTGGTC | ||
SW InfA-Probe | HEX-CYACTGCAAGCCCA“T”(BHQ1)ACACACAAGC AGGCA | ||
HA3-115-F | GCTACTGAGCTGGTTCAGAGTTC | H3 | [19] |
HA3-375-R | GAAGTCTTCATTGATAAACTCCAG | ||
HA3-208-Probe | FAM-CTATTGGGAGACCCTCATTGTGATGG-BHQ1 | ||
Primers for sequence | |||
H1-F | AGCAAAAGCAGGGGAAAATAA | H1 | [20] |
H1-R | GCTATTTCTGGGGTGAATCT | ||
H3-F | AGCAAAAGCAGGGGATAATTC | H3 | [20] |
H3-R | TGCCTGAAACCGTACCAACC | ||
Inf B-F | AGCAGAAGCGTTGCATTTTC | H3 | [21] |
Inf B-R | ACCAGCAATAGCTCCGAAGA |