Table 4 Detection limit of the qRT-PCR assay for Zika virus
From: Quantitative real-time PCR detection of Zika virus and evaluation with field-caught Mosquitoes
Synthetic RNA /μl | nb positive/ nb tested | Ct | Percentage |
|---|---|---|---|
3,2 × 109 | 3/3 | 12.15 ± 0.15 | 100 |
3,2 × 108 | 3/3 | 15.76 ± 0.21 | 100 |
3,2 × 107 | 3/3 | 19.32 ± 0.25 | 100 |
3,2 × 106 | 3/3 | 22.62 ± 0.27 | 100 |
3,2 × 105 | 3/3 | 25.28 ± 0.31 | 100 |
3,2 × 104 | 3/3 | 28.50 ± 0.46 | 100 |
3,2 × 103 | 3/3 | 31.77 ± 0.39 | 100 |
3,2 × 102 | 3/3 | 34.79 ± 0.43 | 100 |
3,2 × 101 | 0/3 | neg | 0 |
3,2 × 100 | 0/3 | neg | 0 |