Skip to main content

Table 4 Detection limit of the qRT-PCR assay for Zika virus

From: Quantitative real-time PCR detection of Zika virus and evaluation with field-caught Mosquitoes

Synthetic RNA /μl nb positive/ nb tested Ct Percentage
3,2 × 109 3/3 12.15 ± 0.15 100
3,2 × 108 3/3 15.76 ± 0.21 100
3,2 × 107 3/3 19.32 ± 0.25 100
3,2 × 106 3/3 22.62 ± 0.27 100
3,2 × 105 3/3 25.28 ± 0.31 100
3,2 × 104 3/3 28.50 ± 0.46 100
3,2 × 103 3/3 31.77 ± 0.39 100
3,2 × 102 3/3 34.79 ± 0.43 100
3,2 × 101 0/3 neg 0
3,2 × 100 0/3 neg 0