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Table 3 Nucleotide sequences of primers and probe used in the qRT-PCR assay

From: Quantitative real-time PCR detection of Zika virus and evaluation with field-caught Mosquitoes

 

Sequence 5′ – 3′

Nucleotide position

Probe

FAM-CTYAGACCAGCTGAAR-BBQ

9304–9320

Forward primer

AARTACACATACCARAACAAAGTG GT

9271–9297

Reverse primer

TCCRCTCCCYCTYTGGTCTTG

9352–9373

  1. FAM, 6-carboxyfluorescein; BBQ, Black Berry Quencher. Y = T or C, R = A or G.
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Virology Journal

ISSN: 1743-422X