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Table 3 Nucleotide sequences of primers and probe used in the qRT-PCR assay

From: Quantitative real-time PCR detection of Zika virus and evaluation with field-caught Mosquitoes

  Sequence 5′ – 3′ Nucleotide position
Probe FAM-CTYAGACCAGCTGAAR-BBQ 9304–9320
Forward primer AARTACACATACCARAACAAAGTG GT 9271–9297
Reverse primer TCCRCTCCCYCTYTGGTCTTG 9352–9373
  1. FAM, 6-carboxyfluorescein; BBQ, Black Berry Quencher. Y = T or C, R = A or G.