Figure 5

Effect of n-butanol fraction prepared from A. catechu on integration of HIV-1 genome in host cells. TZM-bl cells were infected with HIV-1_NL4.3 (MOI, 0.05) pretreated for 1 h with n-butanol fraction prepared from A. catechu (50 μg/ml) and Raltegravir (10 μM), used as a positive reference control and incubated for 48 h in the presence or absence of extract/Raltegravir as described in Materials and Methods. After 48 h, cells were trypsinized and washed. Genomic DNA was isolated from HIV-uninfected/infected cells. DNA (200 ng) was used as a template and viral integration was monitored by Alu-LTR-PCR with Alu-gag primers and GAPDH was used as internal control.