Anti-HIV-1 activity of n-butanol fraction of Acacia catechu using human peripheral blood lymphocytes (PBLs). A) Anti-viral effect of the n-butanol fraction using peripheral blood lymphocytes (PBLs) against HIV-1NL4.3. PHA-P activated PBLs were infected with virus and were treated with different concentration of n-butanol fraction from A. catechu. p24 was estimated on 5th day in the culture supernatant. Significance (p-value) was calculated by comparison with amount of p24 in the infected control group. B) The amount of p24 secreted by infected PBLs in term of percent inhibition presented on the Y-axis. Values are expressed as mean ± SE of 3 different experiments. *p < 0.05; **p < 0.001 (as compared to infected control group without any treatment).