Table 4 Primers used for HPV16 E6 gene and HPV16 LCR amplification
From: Association of human papillomavirus type 16 long control region mutation and cervical cancer
Primers | Sequences (5’ to 3’) | Positions | Product size (bp) |
|---|---|---|---|
E6-1 F | TTGAACCGAAACCGGTTAGT | nt 46–65 | 211 |
E6-1R | GCATAAATCCCGAAAAGCAA | nt 237–256 | |
E6-2 F | GCAACAGTTACTGCGACGTG | nt 204–224 | 235 |
E6-2R | GGACACAGTGGCTTTTGACA | nt 419–438 | |
E6-3 F | CAGCAATACAACAAACCGTTG | nt 371–391 | 220 |
E6-3R | TCATGCAATGTAGGTGTATCTCC | nt 568–590 | |
LCR1-F | GAAAACGAAAAGCTACACCCA | nt 7083-7104 | 286 |
LCR1-R | CAATGAATAACCACAACACAATTA | nt 7345-7368 | |
LCR2-F | GCTTGTGTAACTATTGTGTCATG | nt 7289-7311 | 292 |
LCR2-R | GTGCAGGTCAGGAAAACAG | nt 7562-7580 | |
LCR3-F | ACTTGTACGTTTCCTGCTTG | nt 7525-7544 | 350 |
LCR3-R | GTGTAACCCAAAATCGGTTTGC | nt 7853-7874 | |
LCR4-F | GTCACCCTAGTTCATACATGA | nt 7777-7797 | 231 |
LCR4-R | TGCAGTTCTCTTTTGGTGC | nt 85-103 |