Figure 5

Effects of rapamycin on the oncolytic replication of Ad-cycE. A549 cells were non-treated or treated with 200 nM rapamycin alone, 0.5 MOI Ad-cycE alone, combination treatment of 200 nM rapamycin and 0.5 MOI Ad-cycE. (A) Viral yields were determined at 48 hr p.i. with the infection unit method. The values represent the means ± S.D. of independent triplicate. (B) Cell lysates harvested at 18 hr p.i. were immunoblotted for Ad E1A and actin. Actin was used as a loading control.