Figure 3

Effects of rapamycin on cytotoxicity and autophagy. A549 cells were treated with 0 nM, 100 nM, and 200 nM ramamycin (LC Laboratories, Woburn, MA) and collected at 24 hr after treatment. (A) Cell lysates were immunoblotted for LC3 and actin. Actin was used as a loading control. The values indicate the ratios of normalized band intensities of LC3-II to LC3-I. (B) A549 cells were treated with 0 nM, 100 nM, 200 nM, 400 nM, 600 nM and 800 nM ramamycin. The cell viability % was determined at 72 hr after treatment. The values of cell viability % represent the means ± S.D. of independent quadruplicate compared with the 0 nM-control group.