Figure 2

Selective oncolytic replication of Ad-cycE. (A) WI-38, MCF10A, A549, H1299 and MDA-MB-231 cells were infected with AdGFP, Adwt, or Ad-cycE at 5 MOI. CPE was observed at 48 hr or 72 hr p.i and photographed with an inverted microscope Olympus CKX41. (B) Viral yields produced in WI-38, H1299 and A549 cells were determined at 72 hr p.i. with the infection unit method. The values represent the means ± S.D. of independent triplicate. * P<0.05, Student’s t-test. (C) A549 cells were co-infected with 5 MOI AdGFP and mock-infection, AdlacZ, Adwt or Ad-cycE. All fluorescent microscopy is taken at day 1 and day 2 p.i. with an Olympus IX50 microscope (original magnification of ×100).