Table 1 Primers used for site-directed mutagenesis
Primer | Sequence |
|---|---|
T118M-Forward | 5’-TCATCAACAACCAGCATG GGACCATGCAAA-3’ |
T118M-Backward | 5’-TTTGCATGGTCCCAT GCTGGTTGTTGATGA-3’ |
G145K-Forward | 5’-AAACCTACGGACAAA AACTGCACCTGT-3’ |
G145K-Backward | 5’-ACAGGTGCAGTTTTT GTCCGTAGGTTT-3’ |
G145R-Forward | 5’-AAACCTACGGACCGA AACTGCACCTGT-3’ |
G145R-Backward | 5’-ACAGGTGCAGTTTCG GTCCGTAGGTTT-3’ |
rtM204I-Forward | 5’-GATCCT GATGATGTGGTACTGGG-3’ |
rtM204V-Forward | 5’-GATGTG GATGATGTGGTACTGGG-3’ |
rtM204M/I-Backward | 5’-ACGGACAGCCACACGGTGGGGCTCA-3’ |