Specificity of RT-LAMP. A, Agarose gel electrophoresis showing the specificity of RT-LAMP on amplification of different viruses. B, Visual detection of RT-LAMP amplification products of the different viruses using SYBR Green I staining. The color change from orange in the negative to green in the positive reaction. The lane and tube numbers correspond to the following specimens: Lanes 1, 2, 4 and 5, Akabane virus strain JaGAR 39/MB19; Lane 3, Bovine herpesvirus 1 strain 758; Lane 6, Bovine parvovirus strain BF15; Lane 7, Bluetongue virus strain CSIRO 154; Lane 8, Bovine viral diarrhea virus strain NADL; Lane M, 100-bp DNA Ladder.