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Figure 2 | Virology Journal

Figure 2

From: Classical swine fever virus triggers RIG-I and MDA5-dependent signaling pathway to IRF-3 and NF-κB activation to promote secretion of interferon and inflammatory cytokines in porcine alveolar macrophages

Figure 2

Protein expression of IFN-α,IFN-β,IL-1β,IL-6 and TNF-α was measured by ELISA within 24 hpi following challenge of CSFV in porcine alveolar macrophages. Cells were treated CSFV Shimen isolates at MOI of 0, 0.1, 0.5, 1 or 3. At 24 hpi, cell culture supernatants were collected to analyze the protein expression of IFN-α (A) , IFN-β (B) , IL-1β (C) , IL-6 (D) and TNF-α (E) by ELISA. The experiment was repeated three times and the figure shows a representative experiment. Data is expressed as mean ± SD. An asterisk indicates a statistically significant difference from uninfected cells, * P < 0.05 and ** P < 0.01.

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Virology Journal

ISSN: 1743-422X