Table 1 Virus segment-specific PCR primers and corresponding SNPs
|  | Designation | Sequence 5‘→3’ | Amplicon length (bp) | No. of SNP | SNPs in R65 (top row) versus R1959 (bottom row) = * |
|---|---|---|---|---|---|
Segment 1 (PB2) | R65-PB2-1648Fw | GGTCCTGAGTCAGTGCTTG | 94 | 4 | GAAT |
R65–PB2-1742Rv | CGGTTCAAACTCCATCTTATTGT |  |  | ACGG | |
Segment 2 (PB1) | R65-PB1-1524Fw | ATTTGTAGCCAATTTCAGTATGGA | 128 | 4 | CAGG |
R65-PB1-1652Rv | TGAAGAGCCATCTGAGCTG | Â | Â | TGTA | |
Segment 3 (PA) | R65-PA-1547Fw | GATCCCACTTGAGGAATGATAC | 78 | 3 | TTA |
R65-PA-1625Rv | CAGTACTTTTCCCACTTGTGTG | Â | Â | CCG | |
Segment 4 (HA) | R65-HA 204Fw | ATCTAGAYGGAGTGAAGCCTC | 85 | 3 | GCC |
R65-HA 289Rv | TAAGACCATTCCGGCACATTG | Â | Â | ATT | |
Segment 5 (NP) | R65-NP-355Fw | TGGGTGAGAGAGCTGATTCTGTACG | 113 | 3 | ATT |
R65-NP-468Rv | TGGAATGCCATATCATCAGGTG | Â | Â | GCC | |
Segment 6 (NA) | R65-NA-1156Fw | GGTCAGGATATAGCGGGAG | 84 | 3 | CGC |
R65-NA-1240Rv | ATTAACTCAACCCAGAAACAAGG | Â | Â | TAT | |
Segment 7 (M) | R65-M-119Fw | TTTGCAGGAAAGAACACCGATC | 93 | 2 | CC |
R65-M-212Rv | CAAATCCCAACATCCCTTTAGTC | Â | Â | TT | |
Segment 8 (NS) | R65-NS1-224Fw | GCGAATTCTGGAGGAGGAG | 112 | 4 | CACC |
R65-NS1-336Rv | AAGGGAACCTGTCACTTTCTG | Â | Â | TGAT |