Differential cellular gene expression in duck trachea infected with a highly or low pathogenic H5N1 avian influenza virus

Table 1 Relative amount of differentially expressed genes in H5N1-infected tracheas as compared to control and in HPAI-H5N1- as compared to LPAI-H5N1 infected tracheas using quantitative PCR

Identified gene	Genbank accession number	Fold change at 4 h p.i.			Fold change at 8 h p.i
Identified gene	Genbank accession number	LP versus control^a	HP versus control^a	HP versus LP^b	LP versus control^a	HP versus control^a	HP versus LP^b
TRIM33	XM_418009	0.50±0.21	1.41±1.34	2.84	0.69±0.24	1.19±0.15	1.72*
H-Ras	XM_415752	0.87±0.47	0.17±0.23	0.20**	0.22±0.02	0.15±0.04	0.68**
RABL5	CR406564	1.45±2.23	28.85±34.10	19.85	2.43±1.49	7.06±5.30	2.9
FOS	XM_002200534	3.36±2.89	2.26±1.90	0.67	0.99±0.73	3.04±0.86	3.08*
RhoA	NM_001245622	1.60±1.24	3.96±6.14	0.40	8.29±12.01	3.89±4.41	0.47
EEF1A1	NM_204157	2.18±1.46	0.15±0.03	0.07	1.31±0.17	0.59±0.25	0.45**
DDX3X	XM_002190542	1.27±0.14	0.29±0.14	0.22*	1.07±0.02	0.4±0.25	0.37**
DCN	AF125250	5.95±1.94	0.29±0.23	0.05**	0.21±0.06	0.22±0.09	1.02
IL13	AC233977	13.14±12.99	7.37±5.72	0.56	5.61±3.73	23.89±20.95	4.26

^aFold change is the mean ± SD of gene expression in 4 infected tracheas as compared to control.
^bFold change is the ratio of gene expression in HPAI-infected tracheas as compared to LPAI-infected trachea as indicated.
* p < 0.05 and ** p < 0.1 (two-sample Student t-test); bold data were two-class SAM significant.

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