Evolution and heterogeneity of multiple serotypes of Dengue virus in Pakistan, 2006–2011

Table 3 Genome-wide mutation and selection pressure analysis of Pakistan DENV-3 isolates

Polyprotein position	Gene	Gene position	Wild type	Pakistan isolates (n=6)	Other reports of similar substitutions	Mixed effect model of evolution (MEME) analysis
Polyprotein position	Gene	Gene position	Wild type	Pakistan isolates (n=6)	Other reports of similar substitutions	α	β^-	q^-	β⁺	P value	Log L
951	NS1	178	L	M	India 2003–07 [32, 33] & China 2009^§	1.83	0.36	0.99	1844.02	<0.01	−53.95
2063	NS3	590	K	R	Unique to Pakistan ^£	1.28	0	0.98	42.42	0.58	−17.20
2393	NS4B	151	M	I	India 2003–07 [32, 33] & China 2009^§	1.35	0.16	1.00	0.31	1.00	−9.87
2678	NS5	188	T	A	India 2003–07 [32, 33], China 2009^§, Venezuela 2001-07^§, Puerto Rico 2006^§	0.85	0.38	0.99	707.57	0.21	−35.65
3052	NS5	562	Q	L	India 2003–07 [32, 33], China 2009^§, Colombia 2004^§	1.74	1.00	0	2.65	0.64	−33.19
3385	NS5	895	S	L	Puerto-Rico 2002-03^§, Colombia 2003^§, Vietnam 2006^§	2.00	0	0.99	35.8	0.47	−21.96

^§Directly obtained from the GenBank database.
^£Reported in this study.
Mutations under positive selection and those that are unique to Pakistan DENV-3 isolates are shown in bold.

ISSN: 1743-422X