Evolution and heterogeneity of multiple serotypes of Dengue virus in Pakistan, 2006–2011

Table 2 Genome-wide mutation and selection pressure analysis of Pakistan DENV-2 isolates

Polyprotein position	Gene	Gene position	Wild type	Post-2008 Pakistan isolates (n=5)	Other reports of similar substitutions	Mixed effect model of evolution (MEME) analysis
Polyprotein position	Gene	Gene position	Wild type	Post-2008 Pakistan isolates (n=5)	Other reports of similar substitutions	α	β^-	q^-	β⁺	P value	Log L
130	prM	16	R	K	Sri Lanka2003-04^§ & Pakistan [17]	1.87	0	0.97	91.05	0.15	−35.52
184	prM	70	S	A	Pakistan^£ [17] & Malaysia (sylvatic) [24, 25]	3.61	0	1	134.67	0.05	−41.97
862	NS1	87	T	S	Sri Lanka2003-04^§	1.25	0	0.97	6.49	0.74	−27.76
1160	NS2A	33	I	V	India2001-06^§ & Sri Lanka 1996-2004^§	0	1	0.06	0.33	0.66	−13.61
1243	NS2A	116	I	T	Thailand 1984 [26]	0	0	0.6	2.7	0.42	−40.58
2891	NS5	400	T	A	Sri Lanka 2003-04^§	0	0	0.93	2.85	0.48	−10.8
2953	NS5	462	L	I	India2001-06^§ & Sri Lanka 1996-04^§	1.12	0	0.99	513.74	0.20	−38.62
3107	NS5	616	E	G	India2001-06^§ & Sri Lanka 1996-2004^§	0	1	0.62	0.85	0.44	−9.39
3122	NS5	631	S	G	India2001-06^§ & Sri Lanka 1996-2004^§	0.35	0.45	0.16	1.44	0.23	−32.15
3352	NS5	861	K	R	Unique to Pakistan ^£	0	0	0.98	20.75	0.26	−11.06
3367	NS5	876	N	S	India2001-06^§ & Sri Lanka 1996-04^§	0	0	0.98	19.81	0.19	−11.44

^§Directly obtained from the GenBank database.
^£Reported in this study.
Mutations under positive selection and those that are unique to Pakistan DENV-2 isolates are shown in bold.

ISSN: 1743-422X