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Figure 4 | Virology Journal

Figure 4

From: A novel helper-dependent adenovirus-based cell culture model for Hepatitis C virus replication and production

Figure 4

Infectious HCV production in Huh-7 cells following HDAdJFH1 infection. (a). IFA of the HCV NS3 protein in Huh-7 cells infected with supernatant from HDAdJFH1-infected Huh-7 cells that were collected on day 17 p.i. Huh-7 cells infected with supernatant from HDAdJFH1/GND-infected Huh-7 cells collected on day 17 p.i. served as negative controls. (b). Inhibition of HCV infection by the anti-E2 and anti-CD81 antibody. The infectious HCV supernatants were pre-incubated with the indicated concentrations of anti-E2 or control mouse IgG antibodies for 1 h at 37°C before addition to naïve Huh-7 cells. Huh-7 cells were pre-incubated with the indicated concentrations of anti-human CD81 or control mouse IgG antibodies for 1 h at 37°C before addition of the infectious HCV supernatant. Total cellular RNA was analyzed by RT-qPCR on day 3 p.i.

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