HCV expression and replication in HDAdJFH1-infected Huh-7 cells. (a). Immunofluorescence assays (IFAs) of the HCV core and NS3 proteins in Huh-7 cells on day 3 p.i. HDAdGFP-infected Huh-7 cells served as a negative control. (b). Western blots detecting the HCV core and NS3 proteins in HDAdJFH1-infected Huh-7 cells at 36 h and 72 h p.i. Huh-7 cells transfected with in vitro-transcribed JFH1 RNA served as a positive control. HDAdGFP-infected Huh-7 cells served as a negative control. (c). Northern blots detecting HCV RNA and 28S rRNA in HDAdJFH1-infected Huh-7 cells, HDAdJFH1/GND-infected Huh-7 cells and the HDAdJFH1-infected 293 cells in the presence of actinomycin-D. A group of infected cells without actinomycin-D served as controls. Magnification, ×40 (a).