Endogenously expressed IFN-α was more effective in inhibiting HBV than exogenous IFN-α. (A-C) HepG2.2.15 cells were transfected with pSecTagB-IFN-α or treated with IFN-α2a at the dose of 30 IU/ml or 60 IU/ml,and then RNAs were isolated after 24 h and 48 h. The mRNA levels of HBx, HBs and HBc were quantified. (D) HBV DNA load was quantified by fluorescence real-time PCR. (E, F) HBsAg and HBeAg levels were tested by ELISA. Data represented of three independent experiments and are expressed as the mean ± SD. *P < 0.05: versus IFN-α2a stimulated group.