Some dsRNA sensing receptors were up-regulated after IFN-α over-expression. (A, B) HepG2 cells and HepG2.2.15 cells were transfected with pSecTagB-IFN-α or empty plasmid as previously described. Histogram showed the relative mRNA expression of RIG-I, MDA-5, LGP2, PKR and TLR3 by qRT-PCR after transfected for 48 h. Data are expressed as the mean ± SD from at least three independent experiments. * P < 0.05: versus empty plasmid transfected group. (C) Western blot analyzed the activation of RIG-I and MDA-5 by delivering pSecTagB-IFN-α into HepG2 cells and HepG2.2.15 cells after 48 h. One representative of three independent experiments was shown.