Figure 3
From: The anti-HBV effect mediated by a novel recombinant eukaryotic expression vector for IFN-α
pSecTagB-IFN-α activated JAK-STAT signal pathway and promoted the induction of IFN-stimulated genes. The pSecTagB-IFN-α or empty plasmid was transfected into HepG2 cells and HepG2.2.15 cells at a final concentration of 1 μg/mL and 1.6 μg/mL, respectively. (A) After 24 h, the mRNA levels of STAT1 were measured by qRT-PCR. Data are expressed as the mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01: versus empty plasmid transfected group. (B) Protein levels were evaluated by western blot. One representative of three independent experiments was shown. (C) Western blot analysis of the protein levels of ISG15, OAS-1 and IFIT-3 in HepG2 cells and HepG2.2.15 cells were performed after transfected for 48 h. These experiments were repeated at least three times.