Figure 5From: Chikungunya virus capsid protein contains nuclear import and export signalsMapping of the NES. (A) Various CHIK-CP-EGFP fusion constructs were used to map the nuclear export signal and their localization with N/C found in the nucleus and the cytoplasm and N in the nucleus. (B) HEK293 cells were transfected either with full-length pCHIKV-CP-EGFP, Δ46, Δ118, or an NES mutant (DLAKL to DAAKA indicated by asterisk (* in A) and analyzed by confocal laser scanning microscopy. (C) The ratio of nuclear and cytoplasmic GFP accumulation served as a determinant of nuclear import and export.Back to article page