CHIKV CP contains a CRM1-dependent NES. (A) HEK293 cells transfected with pCHIKV-CP-EGFP were treated with LMB or mock and analyzed by confocal laser scanning microscopy. (B) Graphical representation indicating the nuclear/cytoplasmic ratio of and untreated cells and cells treated with LMB. (C) CHIKV CP directly interacts with CRM1. The pull-down assay was performed using GST-tagged CRM1 or controls with purified CHIKV CP. The latter was detected by an anti-His antibody. Upper and lower panels show GST or GST-CRM1.