CHIKV CP contains an active NLS. (A) Schematic representation of the CHIKV CP NLS-EGFP fusion protein and partial sequence alignment of SFV and CHIKV capsid regions with the NLS determining sequences shaded in gray. (B) HEK293 cells after transfection with eucaryotic expression constructs for the CHIKV CP NLS domain in fusion with EGFP (pNLS-EGFP) or pEGFP as control. The transfected cells were analyzed by confocal laser scanning microscopy. (C) Graphical representation indicating the nuclear/cytoplasmic ratio of NLS-EGFP compared to cells expressing EGFP only.