Sequences of polyomavirus miRNAs. A, Sequence comparison of the different polyomavirus miRNAs. The mature 5p and 3p miRNAs are indicated in red. Remark that for Bpcv1 and Bpcv2 also a 5p miRNA was observed, but no mapping of the sequence was performed. The sequence that was used for probing of this 5p miRNA is indicated in blue. B, Sequence variants observed in JCPyV. A total of 643 JCPyV nucleotide sequences were retrieved from NCBI and aligned using ClustalW algorithm in BioEdit. Relative abundance (%) of each sequence variant was calculated. JCV-miR-J1 sequence from miRBase V20 was used as reference sequence.