Figure 5

DENV induces production of IFNg by CD4⁺T cells and NOS2 by macrophages. Mice were i.c. inoculated with 4 × 10² pfu of DENV-3 (MG20) and the brains of 4 animals per group were collected on the 8th d.p.i. Mice inoculated with uninfected C6/36 cell supernatants were used as controls. The brains were treated with collagenase and Percoll (Sigma-Aldrich) and the percentage of IFNg- and NOS2-producing cells in the various cell populations was analyzed by flow cytometry: (A) IFNg in CD4⁺ T cells; (B) IFNg in CD8⁺ T cells; (C) IFNg in NK cells; (D) NOS2 in macrophages. *P < 0.05 and ** P < 0.001 when comparing the infected mice to the uninfected mice ( A-C ) . ( D) ***P < 0.0001 when comparing the infected versus uninfected WT and the infected Nos2^−/− mice versus the infected WT mice. Statistical analyses were performed using nonparametric two-way ANOVA tests and the Bonferroni post-hoc test.