Detection of inhibitory ability of synthetic compounds against MERS-CoV pseudovirus infection. Compounds (HP-HSA and ADS-J1) and peptides (C34 and T20) were tested for the inhibition of MERS-CoV pseudovirus entry into target NBL-7 (A) and Huh-7 cells (B) at concentrations of 20 and 2.5 μM, respectively. VSV-G pseudotype was included as the negative control. (3) Detection of the potential cytotoxicity of the compounds to Huh-7 cells. The data are presented as mean percentages of inhibition (or cytotoxicity) ± SD of duplicate wells. The experiment was repeated three times, and similar results were obtained.