Effect of TSA on histone H3 acetylation in the JCV NCCR. A. TC620 cells were treated with or without TSA as indicated and ChIP assays performed as described in Methods. The positive control for the PCR was 1/10 of the input extract used in the IPs. MWM – molecular weight markers; α-Ac H3 – antibody to acetyl-histone H3 (Lys9); NRS – nonimmune rabbit serum; No Ab – no antibody added to the IP (beads alone); PCR Neg Con – PCR negative control (no template added). B. TC620 cells were transfected with p65 expression plasmid or control empty vector plasmid in combination with the JCV NCCR (wt or m1) and treated with or without TSA as indicated and ChIP assays performed as in Panel A.