Effect of RNA interference for NF-κB p65 on TSA-mediated stimulation of JCV transcription. TC620 cells were transfected with the reporter plasmid JCVE-LUC (A) or JCVL-LUC (B) and expression plasmid for p65 and/or nontargeting (NT) or p65 siRNA and treated with TSA as indicated, harvested and assayed for luciferase activity. Activities were normalized to untreated controls. The bar represents one standard deviation. Western blots were performed for p65 and Grb2 (loading control) and are shown inset in each panel.