Figure 4

Effect of TSA or coexpression of p300 on JCV transcription in TC620 cells expressing NF-κB p65. A. TC620 cells were transfected with the reporter plasmid JCV_L-LUC and NF-κB p65 expression plasmid and treated with TSA as indicted, harvested and assayed for luciferase activity. B. TC620 cells were transfected with the reporter plasmid JCV_E-LUC (EARLY) or JCV_L-LUC (LATE) and expression plasmid for p300 as indicated, harvested and assayed for luciferase activity. C. TC620 cells were transfected with the reporter plasmid JCV_E-LUC or JCV_L-LUC and expression plasmids for p65 and p300 as indicated, harvested and assayed for luciferase activity. Activities were normalized to untreated controls. The bar represents one standard deviation.