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Figure 5 | Virology Journal

Figure 5

From: A baculovirus-mediated strategy for full-length plant virus coat protein expression and purification

Figure 5

Immunoblot against infected- and non-infected-insect cell extracts using anti-Polh-GarMbFV-CP-6xHis. vAc-polh-6xhis-, vAc-polh-GarMbFV-cp-6xhis-, and vSyn-GarMbFV-cp-infected Tn5B extracts were separated by 12% SDS-PAGE (not shown) and transferred to nitrocellulose membrane. The membranes were treated with the antiserum anti-Polh-GarMbFV-CP-6xHis crystals. (A) Polh-6xHis (lane 1) and chimeric protein Polh-GarMbFV-CP-6xHis (lane 2). (B) Soluble GarMbFV-CP and Polh from vSyn-GarMbFV-cp- (lane 1) and non-infected cells extract (lane 2). Lower bands in lane 2 of figure A are most probably degradation products of the Polh-GarMbFV-CP-6xHis protein.

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