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Figure 2 | Virology Journal

Figure 2

From: A baculovirus-mediated strategy for full-length plant virus coat protein expression and purification

Figure 2

Expression analysis of wild type Polyhedrin and recombinant proteins. AcMNPV-, vAc-polh-6xhis- and vAc-polh-GarMbFV-cp-6xhis-infected Tn5B extracts were separated by 12% SDS-PAGE (not shown) and the proteins transferred to nitrocellulose membranes. The membranes were then treated with specific antibodies anti-Polh (I – upper panels in A and B) and anti-6xHis (II – lower panels in A and B). The anti-Polh detected the wild type Polyhedrin as well as the recombinant protein fused to Polyhedrin. On the other hand, the anti-6xHis detected only the recombinant proteins.

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