Figure 5

PEDV E up-regulates IL-8 expression in IEC cells. (A) The concentrations of IL-8 in GFP-E expressing IEC or control cells (treated or untreated with MG132) culture supernatants were measured by ELISA. Data are mean ± SD and representative of three independent experiments. (B) The effect of PEDV E expression on porcine IL-8 transcription in cultured IEC cells. Total RNA was extracted from cells expressing either GFP alone, GFP-E fusion, or untransfected cells. Real-time RT-PCR analysis of IL-8 mRNA levels were normalized to the corresponding CT value for porcine β-actin mRNA. The results are means ± SD and representative of three independent experiments.