Figure 4

PEDV E expression increases NF-κB activity in IEC cell lines. (A) NF-κB p65 activation was determined by Western blot with the monoclonal antibodies against NF-κB p65 in all cell lines. β-actin was used as an internal loading control. (B) NF-κB p65 activation was determined using the TransAM assay. The data represent the mean and standard deviation from three different experiments.