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Figure 1 | Virology Journal

Figure 1

From: Porcine epidemic diarrhea virus E protein causes endoplasmic reticulum stress and up-regulates interleukin-8 expression

Figure 1

Detection and subcellular localization of GFP-E fusion protein in IEC cells. (A) Western blot analyses of expression products of GFP-E fusion protein in IEC cells. The cells were subjected to Western blot using anti-GFP antibody. Proteins were isolated from whole extracts of the stable cell lines expressing GFP-E protein and control cells. (B) The cells were subjected to Western blot using porcine anti-PEDV antibody. (C) Detection of GFP-E fusion protein subcellular localization in IEC cells by confocal microscopy. All the cell lines were stained by Hoechst33342 and ER-Tracker™ Red. Merged images showed co-localization of GFP-E protein primarily in the ER, with small amounts translocating in the nucleus. Bar = 20 μm for all the figures.

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Virology Journal

ISSN: 1743-422X